The Stanford University Protein and Nucleic Acid (PAN) Facility in the Beckman Center is seeking a Life Science Research Professional (LSRP2) to join our highly multidisciplinary group. The Beckman Center's PAN facility is a multifaceted biotechnology core facility providing access to key tools and applications to researchers through Stanford University and other research institutions, both non-profit and biopharmaceutical. The LSRP2 will work with Stanford researchers to plan and execute genomic technology, and molecular biology experiments as well as participate in other lab activities. The LSRP2 will work independently on multiple projects simultaneously. The LSRP2 should possess an aptitude for learning new technologies to support the needs of a variety of research projects. The LSRP2 must have the ability to recognize irregularities in experimental results and modify protocols as needed. The position requires an individual who is meticulous, highly organized, works efficiently, can prioritize projects and can effectively interact with a large number of diverse researchers from different scientific disciplines. The core facility environment requires this person to be proactive, cooperative, and willing to work with other facility staff as well as other Stanford investigators. Duties include: Responsible for Nucleic Acid QC (Agilent Bioanalyzer, Fragment Analyzer) processing client RNA/DNA samples for Real-time PCR singleplex and multiplex assays analysis, DNA sequencing and Fragment Analysis (ABI DNA analyzers) and Pyrosequencing (Qiagen, PyroMark) using molecular biology procedures. Development of QPCR-based applications to address biological questions including primer design, their validation and optimization in real-time PCR reactions Interpret and analyze results of Nucleic Acid QC, Real-time PCR, DNA Sequencing and Fragment Analysis data often where few guidelines exist and make modifications to existing procedures and protocols. Develop new research protocols where appropriate protocols are not described in the literature or where modification or adaptation of standard procedures and protocols is required. Independently conduct a portion of the research project(s). Make recommendations on experimental design and/or research direction to investigators Perform routine equipment calibration and maintenance where required Troubleshooting of instrument performance and ordering repairs when necessary to meet the advertised turnaround time Work with vendors and customers to implement technical protocols Directly assist members of other teams within the genomics and protein biochemistry groups so that production goals and throughput requirements are met Participate in multidisciplinary teams across different faculties or schools. Perform ongoing literature review to remain current with new procedures and related research; apply to ongoing research and development of new protocols. Compile and maintain records of samples and archive of results in a timely fashion Contribute substantively to the preparation of papers for publication, especially in the results section, and to publication of findings. Present ongoing work to colleagues and/or academic conferences. Oversee supply and equipment budget under supervision of principal investigator or business manager. Work with health and safety department to ensure lab complies with regulations and all required trainings. Supervise (either formally or informally) staff or students as needed, including oversight and direction on techniques, as well as consultation on project work. Serve as a technical resource for other research staff. Oversee the daily activities of the laboratory including lab maintenance; maintain lab stock, manage chemical inventory and safety records, and provide general lab support as needed. May assist with purchasing laboratory equipment. * Other duties may also be assigned
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